Modifications of the PAMONO-Sensor Help to Size and Quantify Low Number of Individual Biological and Non-Biological Nano-Particles

In a series of recently published works, we demonstrated that the plasmon-assisted microscopy nano-objects (PAMONO) technique can be successfully employed for sizing and quantification single viruses, virus-like particles, microvesicles charged non-biological particles. This approach enables label-free, but specific detection biological nano-vesicles. Hence, sensor, which was built up utilizing microscopy, possesses relative versatility it used as platform cell-based assays. However, one challenging tasks such sensor ability to reach homogeneous illumination whole surface gold slide. Moreover, in order enable even relatively low concentrations nano-particles, focused image area had expanded. Both were solved via modifications previously described PAMONO-sensor set ups. Taken together, our latest findings help develop research diagnostic based on principles plasmon resonance (SPR)-assisted nano-objects.